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The remaining parts of all alignments are concatenated to one huge alignment.
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This may improve numerical stability for huge alignments.
FastTree 2 allows the inference of maximum-likelihood phylogenies for huge alignments.
The limit on rounds ensures a predictable running time, but FastTree usually converges before reaching the limit, even for huge alignments such as 237,882 16S rRNA sequences.
We used the huge.1 alignment from the original FastTree paper [ 9].
The improvement can be quite dramatic: for the huge.1.20000 alignment, local search improved the accuracy from 62% to 96%.
As alignment-based methods may be computationally expensive and not scalable to huge datasets, alignment-free methods have been extensively investigated recently.
Based on approximately maximum-likelihood method, FastTree2 was designed to process huge multiple sequence alignments efficiently, using reasonable amount of memory without sacrificing the quality of phylogenetic trees.
The AJAX-based web interfaces in PlantOrDB perform well when displaying the phylogenetic tree and multiple sequence alignment for huge gene families, especially for those with over a thousand gene members.
For every two sequences, there are huge permutations of possible alignments (cubic in the length of sequences).
We present the PUmPER framework and make available a phylogenetic analysis pipeline that can automatically update existing huge reference phylogenies and alignments by new sequence data, without the need to recompute everything from scratch.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com