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These RIDs, including the RID of the primary hub reaction were compared with the list of RIDs of reactions occurring in humans.
An absence of all the RIDs in humans implied that the particular enzyme did not function in the metabolome of humans and the primary hub reaction was then classified as a hub 'unique' to the mycobacterium.
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It is also discovered that both hubs of reactions and the isolated reactions in purine and pyrimidine metabolisms play important roles in T. pallidum.
Reactions in the clusters which have large within-cluster distances are hubs while reactions in the clusters with small but nonzero within-cluster distances are nonhubs or peripheral nodes.
Identification of hubs reveals reactions involving glutamate to be central to mycobacterial metabolism, and pyruvate to be at the centre of the E. coli metabolome.
Ros Atkins spoke to Kate Farrell, editor of World News America, and journalist Andrée Massiah from the User-Generated Content Hub about the reaction to the controversial case.
The approach identifies 139 compounds involved in eight or more reactions as hub compounds and introduces the reactions between the start compound and the hub compounds as the first steps in the search.
The pantothenic acid coenzyme functions in the tricarboxylic acid cycle (also called the Krebs, or citric acid, cycle), which interconnects carbohydrate, fat, and protein metabolism; this coenzyme (coenzyme A) acts at the hub of these reactions and thus is an important molecule in controlling the interconversion of fats, proteins, and carbohydrates and their conversion into metabolic energy.
It was observed that the top 50 degree hubs in the reaction networks of the three organisms comprised reactions involving the metabolite L-glutamate as well as reactions involving pyruvate.
To identify highly connected reactions essential and central to the metabolism of the three organisms under study, we elucidated the hubs in their reaction networks by graph spectral analysis as well as by degree analysis.
To identify hubs in the reaction networks of M. tuberculosis and M. leprae that were absent in the metabolism of humans, we first obtained a comprehensive list of biochemical reactions occurring in the metabolome of Homo sapiens from the KEGG database.
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