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A total of 100 ng cDNA was used for real-time PCR with SyBR Green as indicator using the Applied Biosystem (7900) HT fast real-time PCR system.
Real-time PCR Amplification was performed using the Applied Biosystems 7900 HT fast RT-PCR system.
Reactions were run on a 7900 HT FAST real time PCR system (Applied Biosystems, USA).
QPCR was performed with samples from the heterozygous and wild type males with three replicates each in a 7900 HT Fast Real Time PCR System (Applied Biosystems).
cDNAs (100 ng) were analyzed by real-time quantitative RT-PCR using TaqMan Universal PCR Master Mix Applied Biosystemss) on the 7900 HT Fast Real Time PCR system with a TaqMan low density array (Applied Biosystems).
Real time qPCRs were performed using 15 ng of cDNA per 10 µl using Bio-Rad iQ SYBR Green Supermix in the Eppendorf epMotion 5070 and the Applied Biosystems 7900 HT Fast Real Time PCR System.
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Amplifications were run in a 7900 HT-Fast Real-time PCR system (Applied Biosystems, Foster City, CA, USA).
Quantitative real-time PCR was performed in a 7900 HT-Fast real-time PCR Life Technologiessystemtem with Taqman Universal PCR Master Mix using Taqman assays (Life Technologies) or probes from the Universal Probe Library (Roche, Mannheim, Germany).
Amplification was monitored under standard cycling conditions using a 7900 HT Fast Real-Time PCR System (Applied Biosystems, Foster City, CA, U.S.A.).
To determine mRNA expression levels, real-time quantitative RT-PCR analysis was performed on a 7900 HT Fast Real-Time PCR Systems (Applied Biosystems) using SYBR green fluorescence signals.
Quantitative RT-PCR was performed using the 7900 HT Fast Real-Time PCR System Applied Biosystemss).
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