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Alq3-ethanol solvated phase was annealed at ~200°C under nitrogen atmosphere for 2 hrs to remove ethanol from the crystal lattice.
Briefly, for exosome production, cell culture media containing FCS was centrifuged at 100,000g for 15 hrs to remove any contaminating exosomes.
Feathers were first soaked in a 2∶1 solution of chloroform: methanol for 24 hrs to remove surface oils, then allowed to air dry for 36 hrs.
The films were calcinated at 450 °C for 5 hrs to remove the organic compound.
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To minimize the non-specific effects of Hoechst dye on non-SP cells, we cultured both SP and non-SP cells for 24 hr to remove dead cells and then performed all experiments described below.
Samples were then placed on a freeze dryer for ≥ 3 hr to remove any remaining liquid.
Solubilized membranes were then centrifuged at 200,000× g for 45 min to 1 hr to remove insoluble particulates.
The lipid film was subsequently flowed with nitrogen for 2 hr to remove residual traces of organic solvents.
As in kidney cells [ 24], it took 70 hr to remove all cilia from the epithelium of the osculum.
Cultures were washed three times and treated with 20 ng/ml gentamycin for 2 hr to remove extracellular bacteria.
The incubation mixture was then centrifuged at 105,000 relative centrifugal force (RCF) at 4°C for 1 hr to remove microsomes.
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