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HeLa cells were transfected with PREP1 or Luc siRNAs oligonucleotides, and 48 hrs later they were pulse-labeled (20 minutes) with BrdU.
After 24 hrs, 1 μg/mL dox was added and GFP-Ipr1 expression was measured 24 hrs later using celigo cytometer.
Cellular lethality was assessed by measurement of lactate dehydrogenase (LDH) 24 hrs later.
Hrs later, Romeo hospitalized.
I asked him if he'd be up for a remix, I was more then grateful that he was, less then 24 hrs later, he sent me this mix.
MHEC were harvested 4 hrs later.
The pancreata were removed 6 hrs later, and processed.
24 hrs later, cells were transfected once more.
18 hrs later adoptively transferred mice were sacrificed.
P. falciparum parasites were synchronized with 5% sorbitol and MeSter was added 6 hrs later.
48 hrs later, cell extracts were analyzed by immunobloting with an anti-Kit antibody.
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