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Only socio-economic groups with significant HRs in at least one gender were included.
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All three catalytic TLSP proteins reached maximal levels a few hours after DNA replication (meiotic S), which takes place under these conditions at ∼2 hr in meiosis (at the time of Ime2 induction [ Figure 1B and [ Benjamin et al. 2003]).
Using CTpr at 24 hrs in addition to interleukin-8 at 48 hrs produced the best-fit models associated with sepsis [ 19].
Sperm were incubated for at least 2 hr in HTF++ at 37°C in a 10% CO2 atmosphere.
To simulate the impact of cold ischemia – samples were kept for 1 hr, 3 hrs and 6 hrs in a humidified chamber at room temperature before freezing.
To obtain revertants, the mutant strains were subcultured twice for 24 hrs, in BHI without antibiotics at 25°C.
The culture was allowed to grow for 24 hrs in a shaking incubator at 37°C.
After hybridization, the cells were washed twice in 10% formamide in 2 × SSC for 20 min at 37° and two times 1 hr in PBS at room temperature.
In particular, they were left to recover 1 hr in ACFS at 25°C.
Around 48 hr post-transfection, the cells were incubated with 100 nM Lysotracker (Molecular Probes, USA) in growth medium for 3 hr in dark, at 37°C in a 10% CO2 incubator.
Cells were postfixed in a solution of 1% osmium tetroxide and 1% potassium ferrocyanide for 1 hr in air at room temperature.
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