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The nature and extent of the damage increased up to 48 hrs from the start of the challenge.
Five fish were sampled from each tank at 2, 6, 10, 24, 48, 168 (1 wk) and 504 (3 wks) hrs from the start of the challenge.
Interestingly, there were no mortalities of exposed fish and after 3 wks (504 hrs) from the start of the trial recovery appeared to have been initiated.
At 3 wks (504 hrs) from the start of the experiment there were signs of early stage repair in the gill tissues.
The clinical signs of gill disease for each sampling point detailed in hrs from the start of the experiment were: (a) 2 hrs lamellae with multi-focal areas of epithelial sloughing, necrosis and haemorrhage; (b) 6 hrs increased lamellar epithelial sloughing, oedema and necrosis.
Some interactions are also observed at order-5 or 6 hrs from the start of the experiment.
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After 14 days under SDs, leaf material was harvested every 2 hr for a total of 24 hr from the start of the light period (time point T0).
There was also a significant interaction with time; gill scores from 24 to 504 hrs (3 wks) from the start of the experiment were significantly higher than the first 3 sampling periods (Table 1).
Weighted mean MAP and HR values, recorded from the start of the study drug infusion until 24 hours after discontinuation of study drug, were summarized by treatment group and assessed using analysis of covariance, with the prestudy drug administration value as a baseline covariate.
Animals were withdrawn from food 2 hrs before the start of experiment.
The Cox proportional hazards model was used to estimate the HR and 95% CI of OS/PFS (from the start of treatment).
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