Sentence examples for hrp required from inspiring English sources

Plant infection and colonization by bacterial pathogens require effector molecules delivered into the plant by a type III secretion system encoded by the so-called hypersensitive response and pathogenicity bacterial gene cluster (hrp), required both for HR and disease development [8], [9].

This is exemplified by ELISA experiments, where the extremely densely glycosylated HRP required somewhat higher inhibitor concentrations than ascorbate oxidase (data not shown).

The megaplasmid carries the hrp genes required to cause disease in plants, genes coding for constituents of the flagellum and genes involved in exopolysaccharide production; it also contains 315 genes of unknown function [ 26].

The smaller replicon also contains all the hrp genes (required to cause disease in plants) and it has been suggested that it has a significant function in overall fitness and adaptation of the organism to various environmental conditions [ 26].

Biotinylated or HRP-conjugated isotype-specific antibodies were as for ELISPOT assay (with streptavidin-HRP, if required).

Two master regulators of the hrp systems which are both required for virulence and hrp gene expression in minimal medium have been identified: HrpX is an AraC-type transcriptional activator inducing the expression of all hrp operons but hrpA upon binding to the plant-inducible promoter (PIP) box (TTCGB-N15-TTCGB; B represents C, G, or T) in the promoter region [ 11, 12].

Membranes were then washed with 1X TBST containing 0.1% Tween 20 and incubated as required with the HRP-conjugated anti-goat (Dako, Glostrup, Denmark) or anti-rabbit IgG (Santa Cruz Biotechnology) antibodies.

Originally, the designations "hrp" and "hrc" indicated loci that are required for induction of non-host hypersensitive reaction and for pathogenicity, and individual genes in the loci were given these designations.

The first 14 days of monitoring were omitted from analysis because this period is required to clear circulating falciparum HRP-2 antigen, which the RDT detects from peripheral blood.

While HpaC from Xcv has been shown to be necessary for the secretion of both T3SS effector and translocon proteins, it is not required for the export of the Hrp pilus protein HrpE [55].

Among them, hrpXY encode a two-component system (TCS), which is required for the activation of the hrp gene expression in D. dadantii 3937 [ 30].