Sentence examples for hrp loading from inspiring English sources
The excellent electrochemical performance of the HRP-TNS electrode was attributed to biocompatibility of the titanate sheets, porous architectures of the HRP-TNS film which retained activity of HRP to large extent, avoided aggregation of HRP, provided better mass transport and allowed more HRP loading per unit area.
This indicates that the HRP loading capacity can be controlled by the amount of enzyme present in the reaction.
The facile HRP loading combined with ordered array architecture endows the constructed biosensor with very low detection limit and high sensitivity.
However, the HRP loading capacity of HRP@HSNs gradually increased to 12.5 ± 1.2 μg HRP/mg HSNs when 33.3 nmol of HRP was used (Additional file 1: Table S1).
Compared with nanodisks and nanospheres, the HRP loading on ZnO nanomultipods reached the highest when its glutaraldehyde to amino groups ratio was lower, which indicates that every HRP molecule needs less glutaraldehyde to immobilize HRP on the surface of ZnO nanocrystal and less conformation happened.
To determine the HRP loading capacity, RITC-HRP@HSNs were dissolved in 1 mL of NaOH (1 M) for 1 h, and the amount of the entrapped RITC-HRP was calculated from a calibration curve established by plotting the fluorescence intensity versus the concentration of RITC-HRP.
The mouse anti-GAPDH-HRP (Ab9482) loading control was from Abcam.
For measurement of HRP activity, DCs loaded with HRP-ICs were washed three times with PBS and dispensed (in 100 µL PBS) into microtiter plates (BDBiosciences).
Under the SPE conditions used, we found that ∼60% of the initially loaded HRP could be recovered in the elution step, corresponding to a capacity of ∼24 mg HRP/g particles.
The HRP entrapment efficiency and loading capacity of HRP@HSNs were defined as follows: entrapment efficiency = mass of RITC-HRP in RITC-HRP@HSNs/initial mass of RITC-HRP; and loading capacity = mass of RITC-HRP in HRP-RITC@HSNs/mass of RITC-HRP@HSNs.
Due to the opened structure and lower surface density of amino groups, the loading of HRP on nanospheres is lowest.
