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Furthermore, the growth chamber trial of the parents at 12 and 16 hr light demonstrated that both parents flowered earlier under a 12-hr regime.
Plants were germinated and grown at ~23°C under a 16-hr-light/8-hr-dark regime.
For Arabidopsis, seeds were germinated and grown vertically on Murashige and Skoog medium diluted 10-fold in Petri dishes supplemented with a Pi source of either 500 µM (+P) or 13µMM (-P) NaH2PO4 in a culture chamber under a 16-hr light/8-hr-dark regime (25°C/22°C), as previously described (Misson et al., 2004).
Plants were grown for 44 days in a controlled environment room maintained at 20/15°C day/night temperature and 14/10 hr light/dark regime.
Inbred male mice with germline deletions of either Spry1 [ 29] or Spry2 [ 30] were housed under controlled temperature and 12 hr light/dark regime with food and water provided ad libitum.
HS was imposed by increasing the temperature for two days at 30°C and 22°C in the 15 hrs light and 9 hrs dark regime, for an initial adaptation phase, then the temperature was increased to 34°C and 26°C light/dark for two days and subsequently to 40°C and 32°C light/dark for an additional day when the flag leaves were collected.
WT Arabidopsis thaliana ecotype Columbia (Col-0), an AtPAP2 T-DNA insertion mutant (Salk_013567) and two AtPAP2 OE lines (OE7 and OE21) were grown in soil under a 16-hr light (22°C /8-hr dark (18°C) regime (long day, LD) at a light intensity of 122°C /8-hrl m-2 s-1[ 6].
Briefly, control and transgenic tobacco seeds were sown on solid MS media [ 57] in Petri dishes and incubated at 26°C with a 16 hrs light / 8 hrs dark photoperiod regime.
Plants were seeded in 15.24 cm (6 inches) diameter pots in standard potting media (Seedling Mix No. 1, OBC Northwest, Canby, OR) and cultivated in greenhouses at 20 30°C temperature and 16/8 hr day/night light regime.
Seeds were stratified at 4°C for 2 days in the dark and grown on vertically orientated ½ Murashige and Skoog (MS) medium plates under a long-day regime (16 hr light/8 hr dark) at 20 22°C.
Plates were then moved to a growth chamber and grown under a regime of 16 hr light/8 hr dark at 21° in a growth chamber.
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