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If growing cells were allowed to attain their 1st CP normally, but then treated with zeocin from the 8th hr onwards, all further CPs were abolished (Fig. 3G).
From 4 hr onwards, males showed no significant preference between mated and virgin females.
From 2 hr onwards, the amino acid levels were always lowest in the double mutants (vps4∆, atg8∆).
DOI: http://dx.doi.org/10.7554/eLife.04872.012 Induction of PPP1R15A was observed in thapsigargin-treated cells from 2 hr onwards but was diminished in cells co-treated with jasplakinolide (likely, a consequence of profound attenuation of protein synthesis).
Wing discs were dissected from first instar (24 30 hr AEL), second instar (48 55 hr AEL) and third instar larval (72 hr onwards) stages and then fixed in 4% paraformaldehyde diluted in Phosphate buffered saline (PBS pH-7.5).
There was a suppression of this increase from 72 hr onwards in As + Ars Alb-30 group (p < 0.05), while the increased enzyme activity appeared to be consistently suppressed at all the fixation intervals in the As + Ars Alb-200 group (p < 0.01).
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For both P1 and P2, we estimated competitive fertilization success separately based on eggs laid 0 - 24 hrs (P) and 24 hrs onwards (P) after the second mating, as well as the sum of the two (PT).
Direct-nano administration did show significant drug localization in blood right from the time points 4 hrs post-administration onwards.
The cut off values for blood spot TSH used was 40.0 mIU/L upto 48 hrs (i.e., Day 2) and 20.0 mIU/L after 48 hrs of life (i.e., Day 3 onwards).
Only 9.2% (n = 7090) of babies' blood spots were collected after 72 hrs of life (i.e., from Day 4 onwards).> The sample collection ages ranged from 12 hours of age to 83 days of age.
However, nevirapine did not cause developmental arrest being added to early zygotes (the first 5 hr after fertilization) and later embryos (from the eight-cell stage onwards) [ 35].
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