Electrophoresis was performed using a denaturing 1% agarose gel at 15mA for 3 6 hr (3 hr for Myosin and 6 hr for Titin) on a Hoefer (Holliston, MA) SE 600 gel unit (Steffen, et al., 2007; Warren, et al., 2003).
Crude HRs and HRs adjusted for the potential confounders described above were estimated from the Cox regression analyses.
Body temperature was recorded under LD 12 hr:12 hr for one week followed by DD 12 hr:12 hr for three more weeks.
The respective exposure values at 2 hr were calculated as a linear change (Δ = 2 hr – 0 hr) for each participant.
After 24 hr (20 hr for neutrophils and monocytes) all cells except keratinocytes were stained with propidium iodide (PI) and cell death analyzed on a FACScalibur instrument (BD Biosciences).
All lines were maintained under common conditions (22°, cornmeal-molasses medium, light:dark cycle of 14 hr:10 hr) for more than 10 generations before RNA extraction.
Error bars represent standard deviations from two trials for no irradiation, 2 hr, 8 hr, 24 hr and three trials for 4 hr for both genotypes.
The cell cultures were allowed to continue growing for 6 hr, 8 hr and 10 hr (according to the experimental design) after induction and adjustment of growing temperature.
The cleavage reactions were carried out for 1 hr, 2 hr, 4 hr or overnight (20 ± 1 hr) at 37°C.
Sequential parallel samples were processed at 15 min, 30 min, 1 hr, 3 hr, and 6 hr for total RNA or NRO RNA isolation and labeling.
