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At 20.8 hpf, it concentrates at the equator (white arrow).
At 72 hpf, it has increased in size and starts to spreads over the myocardial surface of the looped heart.
Quantitative analysis shows that although most evagination is complete by 12 hpf, it continues for ∼4 hours (Fig. 2D-H).
Although Vegfc is expressed in the DA at 48 hpf, it is expressed in the hypochord at an early stage (18-somite stage) (Covassin et al., 2006).
At 12 hpf, neurogenesis has just begun, by 18 hpf it is well underway, and by 27 hpf this process is widespread throughout the nervous system.
After 28 hpf, it may not necessarily be appropriate to call the ventral projecting motoneuron axons CaP axons because interpretations in the literature suggest that znp1 only can be used to detect CaP axons prior to 28 hpf.
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Once the computer identifies the HPFs, it is up to the pathologist to select which computer-classified HPFs they want to inspect more closely.
Expression of sox2 from 24 hpf made it the earliest marker for swimbladder epithelium progenitors, which were previously defined at 28 hpf by pbx1 [25] and prdc [31], and at 36 hpf by hb9 [26].
In contrast, hypoxia had little or only modest effect on the IGFBP-1b mRNA expression in early embryos (0 and 24 hpf), but it had a strong effect in later stage (72 and 96 hpf).
Zygotic activation of both wnt8 and runt-1 occurs at late cleavage stage (∼6 hpf), so it was of interest to examine whether the initial expression of wnt8 is dependent on SpRunt-1.
The expression is very pronounced between 20 and 40 hours post-fertilization (hpf), when it marks the hatching gland.
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CEO of Professional Science Editing for Scientists @ prosciediting.com