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Purified HPCs (1 × 10 cells ml−1) were grown in fetal calf serum free (FCS−) unilineage MK liquid culture [ 26], in the presence of saturating doses of thrombopoietin (TPO) (100 ng ml−1) (Peprotech, Rocky Hill, NJ, USA) alone or in combination with aspirin (50 μ m) or WY14643 (1 μ m).
Similar(59)
IL-6 enhances proliferation of HPCs [44].
IL-6 stimulates proliferation of early hematopoietic progenitor cells (HPCs) [16].
Our results showed that we were able to reconstitute the Rag-deficient mice with ES cell-derived HPCs [17].
The CD34+HPCs population is an heterogeneous population containing: i) very few HSCs (<0.1%), ii) minority of pluripotent HPCs (1-3%) and iii) majority (>90%) of committed progenitors (i.e. BFU-E, BFU-MK, CFU-GM).
Besides HSCs or HPCs, GATA2 also regulates hematopoietic lineage specification.
However, the ratios of total CD45+CD34+ HPCs, CD45+CD34+ CD38− immature HPCs and CD45+CD34+CD38+ less immature HPCs among the total CD45+ cells were unchanged (Fig. 4D).
A similar signature was also observed to be present in haematopoietic progenitor cells (HPCs) (48).
Exponentially growing HPCs (1.0 × 10) in DMEM (20.0 mL) were incubated with each monomer for 4 and 24 h.
Above the root tip in HPCs, PDR1 was present in the outer-lateral plasma membrane, compatible with its postulated function as SL exporter from root to soil.
One candidate gene is 2′-5′-oligoadenylate-dependent RNase L (RNASEL), which is located in the hereditary prostate cancer (HPC) 1 region (1q24-25).
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