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The NK cell product was collected by leukapheresis 5 days after the second HPC collection.
Following induction, patients underwent HPC collection either with G-CSF alone, or G-CSF and cyclophosphamide (Cy) for mobilization.
38– 41 Mark et al 42 evaluated the efficacy of cyclophosphamide in overcoming the suppressive effect of lenalidomide on HPC collection in myeloma patients.
43 Recently, the International Myeloma Working Group IMWGG) published guidelines for HPC collection following initial therapy with thalidomide-, lenalidoride-, or bortezomib-containing regimens.
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The topics covered by this chapter include evaluating the manufacturing of various hematopoietic progenitor cells (HPCs) products, reviewing the donor eligibility/suitability for HPCs collections, and also understanding the unique challenges of HPC mobilization/collection.
In addition to progenitor cells, PB-HPC collections contain other hematopoietic cells.
Although the PBHPC collection procedure is designed to enrich for mononuclear cells, all PB-HPC collections contain granulocytes, erythrocytes, and platelets.
Much variation exists in data collection around HPC delivery.
miRNAs selected for validation are represented in bold miRNAs selected for validation are represented in bold The recognition that PC clusters within families has led to the collection of HPC families with the goal of localizing and identifying PC susceptibility genes.
Approximately 33% of patients who received G-CSF alone were not able to collect adequate HPC; however, all of the patients who received Cy had successful collection (P < 0.0001).
That was done partially because we had access to the database schemas of the seven HPC centres and also because we believe HPC data collection needs to move towards electronic collection.
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