Sentence examples for hp buffer from inspiring English sources

Once thawed, suspended filaments were sedimented (500 ×  g, 5 min, 4°C), resuspended in 50 ml of N2-sparged HP buffer (30 mM Hepes/30 mM Pipes/1.0 mM MgCl2, pH 7.2), and washed three times with N2-sparged HP buffer containing 10 mM disodium ethylenediaminetetraacetic acid (HP/EDTA).

It uses two schedulers: an input scheduler aims to prioritize video streams with superior quality (HD and SD) and consists of a high-priority (HP) buffer for, e.g., UGS, ertPS, and rtPS, and a low-priority (LB) buffer, which includes rtPS-web-TV, rtPS-mobile-TV, nrtPS, and BE.

100 mM HEPES/KOH, pH 7.2 with 100 mM NaCl (HP buffer) was used for maturase extract dialysis.

Reconstituted protein solutions were centrifuged for 15 min at 8,000×g and passed through PD 10 desalting columns (GE Healthcare) equilibrated with HP buffer.

Phase 1, dialysis: 0.5 2.0 mL of maturase extract was buffer exchanged three times (3 hr, 3 hr, overnight) against 0.75 L of HP buffer at 6°C using 6 8 kD MWCO RC dialysis tubing (Spectrum Laboratories, Inc .. Dialyzed maturase extracts were used immediately to avoid variability from freezing and thawing.

The supernatant solution was discarded, and the heterocyst-containing pellet was washed three times with HP buffer.

The lysate was clarified by centrifugation and applied to a 10 mL Ni-Sepharose HP column equilibrated against buffer A. The column was washed with 10 column volumes of buffer A, and bound proteins were eluted with 3 column volumes of buffer B [50 mM HEPES (pH 7.8), 150 mM NaCl, 20 mM imidazole, and 10% glycerol].

Filtered lysates of cells expressing SnoN137 238 were loaded onto HiTrap Chelating HP columns (GE Healthcare) in buffer 1 (30 mM HEPES pH 7.5, 500 mM NaCl, 10 mM imidazole, 10% glycerol, and 0.5 mM TCEP).

The sample was passed through a HisTrap HP column equilibrated with buffer A to remove BirA.

Residual His-tagged proteins were removed by passing the sample through a HisTrap HP column (GE Healthcare Life Sciences) in buffer A4 supplemented with 40 mM imidazole.

His6 Cas8′ fractions were pooled and loaded on to 5 ml of heparin HP column equilibrated in buffer C. His6 Cas8′ eluted in a gradient of 150 1500 mM NaCl and fractions containing His6 Cas8′ were pooled and dialysed into buffer D [20 mM Tris/HCl, pH 8.0, 500 mM KoAc, 1 mM DTT, 0.1 mM PMSF and 40% (w/v) glycerol] for storage in aliquots at −80°C.