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Fifty microliters of Blunt/TA ligase master mix (New England Biolabs), 10 μL adapter mix, and 10 μL HP adaptor were added to each dA-tailed DNA and incubated at 20°C for 10 min.
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Twenty-microliter reactions contain 4 μl of dA-tailed DNA (0.2 pmol), 5 μl of adaptor mix (1 pmol) (SQK-MAP004), 1 μl of HP adapter (1pmol) (SQK-MAP004), and 10 μl of Blunt/TA Ligase Master Mix (NEB, cat. #M0367S).
According HP genotypes, individuals were allocated into the HP /HP, HP /HP, and HP /HP groups.
HP: Delia.
HP: Richard Rogers.
HP: Food Processing Technology.
HP company".
A power adaptor.
From HP Lovecraft, mainly.
HP: A gilded sausage.
HP: It's unpleasant.
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