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This study employed a 3×3 full-factorial design of HP (no HP, 5 MPa static, 10 MPa static) and growth factor application (no GF, BMP-2+IGF-I, TGF-β1) for a total of nine treatment groups.
Eluted 6xHisSumo-Int was then run through a HiTrap Heparin HP 5 mL column, and pooled fractions were run on a Superose 6 Increase 10/300 GL column on an AKTA Pure 25 L system (GE Healthcare).
The probe is a "HP" 5 mW, wavelength tunable, CW laser beam around 1550 nm (model "HP 8168F") with 2 mm diameter.
The pooled fractions from Ni-IMAC were dialyzed against 30mM TRIS, pH 8.0 and then loaded on to a HiTrap Q HP 5 ml column (GE) to further purify the two forms of recombinant SAP.
To verify that the predicted stem-loop might exist in vivo and could be involved in differential regulation, two mutated versions of the hp 5'-UTR with either increased or decreased stability were constructed.
The supernatants were loaded on a Hitrap Chelating HP 5 ml column, which were charged with chelated Ni2+ and equilibrated with Buffer A for the first step of purification.
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Recombinant protein from SELP-1020-A SELP-1020-A SELP-1020-A IMAculturea HisTrap HP 5-ml column already prepacked wash a Ni Sepurified High Performance matrix.
The pumping station contained three 100 inches pumps, each powered by a 900 hp 5-cylinder vertical diesel engine manufactured by Ruston.
In the absence of other sources of lipid peroxides and HP, 5-LO is completely inhibited by zileuton.
Histidine-tagged VacA protein was purified by immobilized metal ion affinity chromatography (IMAC), using nickel-sepharose HisTrap HP 5-mL columns (GE Healthcare, Sweden).
The reaction mixture then was passed through a HisTrap HP 5-ml column (GE Healthcare) to remove the 6× His-tagged HRV-3C and any uncleaved protein.
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