In the past few years, however, empirical data have emerged from experiments that were specifically designed to tackle the question of how specificity is encoded by calcium.
Because most of the PRC complexes (except PhoRC) lack specific DNA binding components, an important issue is how specificity in recruitment to loci is achieved and what components are involved.
However, to date, there is no experimental support for a mechanism whereby specific miRNAs are loaded into MVBs for secretion; neither is it known how specificity is achieved in apoptotic bodies and shedding microvesicles.
Knowing how specificity is controlled, would allow to optimise this activity in an efficient way by means of enzyme engineering.
This work provides clues toward uncovering how specificity is achieved within the binding pocket of a polyspecific transporter that may open new possibilities as to how these transporters can be manipulated to bind a designed set of drugs.
Given that, such a large number of stimuli can activate NFκB, which in turn activates an equally large number of target genes, understanding how specificity generated within the framework of pleiotropic signaling is a major challenge.
A multiplatform analysis of gene expression in wheat polyploids highlighted the issue of how specificity among different platforms may affect microarray results [34].
It is less obvious how specificity can be achieved for kinases like FAK that do not natively utilize unique conformations in proximity to the active site for regulation [4].
The BED Capture Enzyme Immunoassay, believed to distinguish recent HIV infections, is being used to estimate HIV incidence, although an important property of the test – how specificity changes with time since infection – has not been not measured.
It remains to be determined exactly how specificity of binding is achieved by ZNF274 but possibilities include heterodimerization with another protein or selective use of a subset of the zinc fingers.
