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Information regarding how samples were collected and processed prior to the acid digestion (described in supplemental materials) is of interest.
In his study, Heer et al (1998) makes no mention of how samples were collected or his method for serum separation.
Often, this performance is a consequence of the testing conditions including how the clinical sample is collected and the level of operator's experience with the test.
What type of sample is collected?
Given the inherent conflict between the desire to collect material in a uniform and optimal manner and the constraints of working in a clinical environment where such collection is not always feasible, the description of how each sample was collected, handled and stored, will be very important.
Data were also recorded about when and how the urine sample was collected (cystocentesis, urinary catheter or midstream catch), when the Flexicult Vet A plates were incubated and read, and who read them.
10,000 events per sample were collected.
Variation could have occurred in the way survey questions were asked or how environmental samples were collected.
Where and how these samples are collected depend on the biologic sample, the chemical of interest, and the age of the participant.
It is also true that data on serum potassium in mice vary significantly depending on how blood samples are collected (after killing versus alive but under anaesthesia).
In this data note we describe how the samples were collected, processed for BS-seq and RNA-seq library construction and analysis, as well as subsequent data mining.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com