Selection of either β-actin or PBGD as housekeeping genes was necessary, because the copy number of the housekeeping gene should be in a similar range with that of the target gene to make comparative quantification possible [ 8].
Housekeeping genes should be generally highly expressed and stable, meaning not variable, across samples.
Data regarding batch variability should also be recorded and reported Common reference RNA standards (housekeeping genes) should be adopted to facilitate comparison between array platforms.
[BIII] Sufficient housekeeping rooms/closets should be provided throughout the facility to maintain a clean and sanitary environment.
The 3'/5' ratios for housekeeping genes should be at most 3 when one-round amplification is performed, and at most 10 for two-round amplification procedures.
Exogenous proteins, labelled analogues or housekeeping proteins should be used as internal standards to assess methodological repeatability and reproducibility (Domon and Aebersold, 2010; Meng and Veenstra, 2011; Maiolica et al., 2012; Picotti and Aebersold, 2012).
For example, a number of housekeeping genes should be satisfactory controls for comparisons of transgene expression level in the same tissue type from different transgenic lines grown under identical conditions.
Based on a more limited MLST approach, using partial gene sequences only as well as a different set of genes than we applied in our MLSA analysis, Cardazzo et al. [ 28] concluded that reticulate evolution of housekeeping genes should be an important factor of B. cereus evolution.
However, it has been reported that housekeeping genes can have variable expression under different systems; hence, the stability of housekeeping gene expression should be validated for each experimental system (Bas et al. 2004; Huggett et al. 2005).
No hats!" He discussed various housekeeping items: clothes should be left in places where you could find them again; people should lie on the ground when instructed.
