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GAPDH was used as the housekeeping reference gene.
Optimised primers for 18S rRNA provide a valid housekeeping reference gene.
β-actin was used as an internal housekeeping reference.
RpS20 was used as a housekeeping reference gene (see ref [7]).
RPS24 was used as the housekeeping reference gene in all cases.
GAPDH was used as a housekeeping reference gene for normalization purposes.
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A confounding issue is the use of the terms "housekeeping" and "reference" genes; housekeeping genes are often used as reference genes, although for many of these individually it was shown they are not good reference genes.
My habits, whether in cooking or housekeeping, directly reference these three women.
The statements about former Miss Universe Alicia Machado, which included calling her "Miss Piggy" for her weight and "Miss Housekeeping" in reference to her Hispanic heritage, were brought up by Hillary Clinton in Monday's debate.
In what has been billed as the most-watched debate in presidential history, Democratic candidate Hillary Clinton made Trump's remarks to Machado a centerpiece of their first televised clash, citing the name-calling – in particular, Trump calling her "Miss Housekeeping" in reference to her Latina origins – as a prime example of her opponent's demeaning views about women.
For accurate and reliable gene expression analysis, normalization of gene expression data against housekeeping genes (reference or internal control genes) is required.
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