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GAPDH antibody was used to determine the housekeeping of each sample (mouse anti GAPDH, 1 : 500, Chemicon).
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The seven housekeeping alleles of each of the 178 isolates yield a total of 80 distinct allelic profiles, referred to as sequence types (STs).
This may be due to coherence imposed by recombination, or it may represent co-evolution within the housekeeping genes of each species group, which could help to maintain distinct groups if hybrids that contain sequence diversity of more than one group are less fit for host-to-host transmission.
They have followed in the footsteps of their baby-boomer parents and, lacking the good housekeeping of older generations, have continued to party long past 1999.
A practice like that seems worthy of more than two paragraphs in a book that devotes so many paragraphs to the slipshod cooking and housekeeping of Aunt Phyl.
Thus, most of the energy is spent on synaptic activities leaving very little energy for housekeeping of neurons.
Generic NICEON technology was compatible with a variety of constitutive viral and mammalian housekeeping promoters, each of which enabled specific induced, repressed, adjusted and reversible transgene expression profiles in Chinese hamster ovary (CHO-K1), baby hamster kidney (BHK-21) as well as in human fibrosarcoma HT-10800) cells.
Relative gene expression levels were calculated based on the ratio of the mean of housekeeping signals of all experiments.
Our results for sequence typing of housekeeping genes of serogroup X meningococci also fit this pattern.
For this purpose we estimated the number of cells present in a given sample by real-time PCR quantification of the copy number of the housekeeping gene GAPDH, each cell having two allelic copies of this gene.
Briefly, to assess antigen-specific gene induction, results for each target gene were normalized by dividing the amount of the amplified gene target by the amount of the housekeeping gene for each sample.
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