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Many 'in house' methods based on reverse-transcribed PCR (RT-PCR) could be difficult to use in the routine diagnostic laboratory since they tend to be time-consuming, use reagents from many different suppliers and include non-routine procedures.
Potential NAA techniques have been evaluated in developing countries, as these methods are more affordable; these in house methods frequently use the IS 6110 element[ 7- 13].
We found no significant differences in the performance of our two "in house" methods, minisequencing with primers directly immobilised on the microarrays (Method I)[ 18] and the "tag-array" format, based on cyclic minisequencing followed by capture on microarrays using immobilised complementary "tag" probes (Method II) [ 23].
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To have accurate results we have chosen two different methods for GYPB*S/GYPB*s genotyping: the allele-specific AS-PCR, an "in house" method, and the HEA (i.e. Human Erythrocyte Antigen) BeadChip, a microarray method commercially available.
PTH (parathyroid hormone) was measured by an immunofluorimetric in house method (IFMA, VR: 10 55 pg/mL) [ 11].
Luckily, my questions about in-house methods for getting babies to cry prompted more mixed responses (take away a toy, take away the mother, "it just happens").
Validated against established in-house methods, the changes made to harmonize analytical protocols did not significantly affect measurement of any of the analytes.
The modified multi-analyte approaches were first validated by comparing data obtained for LS Brown snus using the approaches described above with data previously obtained for this product using established in-house methods.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com