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We used the method developed by Stekel et al. [17] for comparing digital expression patterns, and calculated a R statistic for each cluster, using an in-house program available upon request.
CG, CHG and CHH profiles (where H is A, C or T) were detected using an in house Perl script available upon request.
The tBLASTx results were parsed and formatted using an in-house Perl script (available upon request).
Both, local and remote, searches were automated using in-house Perl scripts (available upon request).
Alignment of the unisequences to repetitive elements in RepBase release 14.08 [ 74] was done with BLASTN that was automated using in-house Perl scripts (available upon request).
Genomic properties such as genome size, AT content and relative entropy were estimated using in-house scripts that are available upon request.
The in-house developed Perl scripts are available upon request.
These analyses were made using in-house perl scripts, which is available upon request.
This process was automated using in-house Perl scripts, which are available upon request.
Randomized networks were obtained using an in-house PERL program, which is available upon request.
Stochastic versions of the models were simulated using in-house developed software, which is available upon request.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com