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We spent six distressing hours at the airport trying to sort out the situation, then three hours transferring to another airport for the next available flight, thus missing a pre-paid night in our French hotel.
Getting There From Pennsylvania Station, the Long Island Rail Road has trains to Oyster Bay roughly every two hours, transferring at Jamaica and scheduled to take about 81 minutes altogether.
Crews had spent hours transferring the shuttle to a special, lighter towing dolly, which was then pulled across by a pickup truck.
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If you must keep them in the freezer longer than 2 to 3 hours, transfer them out of the freezer and into the refrigerator so they loosen up before serving.
Cells were treated for 24 hours or 72 hours, transferred on an enzymatic 96 wells plate and co-incubated with MTT[3- 4,5-dimethylthiazol-2-yl -2,5-diphenyltetrazolium broMTT[3- 4,5-dimethylthiazol-2-yl -2,5-diphenyltetrazolium
To test HSF-1 phenotype dependence, animals were subjected to a 35°C heat shock for 5 9 hours, transferred to 20°C for recovery and scored for dead animals 12 hours later [31].
Jejunal sections were obtained and immediately fixed in 2.5% glutaraldehyde in 0.1 mol/L sodium cacodylate buffer (pH 7.4) for 2 hours, transferred to sodium cacodylate buffer and stored at 4°C overnight.
Digested DNA fragments were separated by gel electrophoresis in 0.8% agarose gel at 60 V for 3 hours, transferred via overnight Southern blot onto a nylon membrane and cross-linked onto the membrane using a UV cross-linker (Stratagene, USA).
Eyes separated from connective tissue after perfusion fixation with PBS containing 14% paraformaldehyde were post-fixed for several hours, transferred to 30% sucrose overnight (4°C) and embedded in Tissue-Tek (Sakura, Heppenheim, Germany).
The brains were fixed with 2.5% Paraformaldehyde (PFA) for 2 3 hours, transferred to 15% sucrose solution with 1% PFA, and incubated at 4°C for not less than 16 hrs.
Kidneys were fixed in Bouin's solution for at least 2 hours, transferred into 70% ethanol, and processed into paraffin.
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