Your English writing platform
Discover LudwigExact(9)
All samples were collected and processed within four hours of expression.
After 24 hours of expression, cells were harvested by centrifugation at 5000 g for 20 minutes, and stored at −20°C for future use.
After four hours of expression, the cells were plated to medium lacking histidine to select for recombinants, and dilutions plated to YPD to determine viability.
After four hours of expression, appropriate dilutions of cells were plated to YPD agar to determine viability after HO cutting, and SC-His to select for recombinants.
To confirm this observation, we used a time course experiment to monitor CFU formation during eight hours of expression and eight hours of recovery.
In transduced myoblasts significant conversion to the LC3b-II form was observed after 16 24 hours of expression of YFP-hDMPK A, but not C (Figure 4D).
Similar(51)
In addition, a 1 µl sample from each cell type was also removed after each hour of expression, diluted 1000-fold into sterile water, and spotted as a series of four 10-fold dilutions onto LB-agar plates that either contained or were devoid of arabinose (1 mg/ml).
Finally, an intriguing fly-to-fly variability in reporter expression patterns was observed: the hsp70 and hsp22 transgenic reporters consistently exhibited ~24 hour or ~18 hour periods of expression, respectively.
After 40 hours detection of expression was conducted by laser scanning confocal microscopy using 488- and 633-nm excitation and two-channel measurement of emission, 522 nm (green/GFP) and 680 nm (red/chlorophyll).
In non-CD4 cells treated with TSA for 4 hours levels of expression of CD11c were increased.
Our new findings demonstrate that INPP4B expression suppresses BIRC5 at the protein level within 24-48 hofrs of INPP4B expression.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com