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The experiment was performed in a 96 well plate with approximately three hours of assay time.
In contrast, SH2-PLA requires only a few microliters of lysate and 6 7 hours of assay time, making this assay highly flexible.
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All odorant dilutions were made within 24 hours of the assay.
The cumulative release of approximately 670 µM of tenofovir from the tenofovir gel occurred over the six hours of the assay.
When used, STS was applied at a 4 μM concentration for the last 2.5 hours of the assay.
I had had a miserable postdoc experience: I did not publish, and I could not embrace the long hours of repetitive assaying.
So far as possible, 2.5 cc blood samples were drawn every 20 min. for 24 hours for assays of luteinizing hormone.
For capillary morphogenesis assays involving taxol or nocodazole, cords first were allowed to form for 3 h and then these agents were added, where indicated, for the final hour of the assay.
We therefore used confocal microscopy to examine the T cells in the first hour of the assay as they migrated over the epithelium.
This diapedesis, to the apical side, takes several hours, and during the first hour of the assay, T cells move over the basal epithelium, but very few T cells cross to the apical epithelium until after 90 min (9).
It was verified that the PenG release occurs in at least two steps: first, a fast release of about 5% in the first hour of the assay, which corresponds to PenG being physically entrapped in the bead's external layer, and second, the PenG being gradually released, reaching 65% in 432 hours.
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CEO of Professional Science Editing for Scientists @ prosciediting.com