Sentence examples for hours in presence of from inspiring English sources

Cells were cultured for 24 hours in presence of 10% serum and processed for pMSK immunofluorescence.

For intracellular IFN-γ, cells were treated with exosomes for a total of 12 hrs with the final 6 hours in presence of 2 µM monensin.

Primary fibroblasts and SKNBE cells were incubated for 48 and 72 hours in presence of 100 µM H2O2 (Sigma Aldrich) or DMEM alone as control before total RNA and protein extraction.

Wif1 incubation for 48 hours in presence of 1% DMSO leads to a significant 42% reduction of the Top/Fop ratio and completely abolished the increase in the Top/Fop ratio at 96 hours.

For intracellular IFN-γ staining of lymphocytes from lung, spleen, and MLN of exosomes treated and untreated mice were incubated with BCG lysate (10 µg/ml) for a total of 12 hrs with the final 6 hours in presence of 2 µM monensin.

The remaining PBMC were cultured for 48 hours in presence of control microvesicles, AT-2 HIV or AT-2 HIV plus 1mT at the concentrations described above, while the isolated CD4+ and CD8+ cells were maintained in RPMI 1640 (Invitrogen,) with 10% fetal bovine serum (Hyclone) and 1% Pen-Strep-Glut (Invitrogen).

Cells were then placed at 37°C and 10% CO2 for 30 minutes, washed with DMEM without antibiotic, and subsequently incubated for 1 hour in presence of gentamicin (50 µg/ml).

(B) samples from gecko (T. mauritanica); lizard (A. Carolinensis); snake (T. elegans; mouse NIH3T3 were stimulated for 10 min prior to lysis (lane1) or were stimulated one hour in presence of MEK inhibitor PD184352 (lane2) to block ERK phosphorylation by MEK Kinase.

On agar plate, the activity was detectable after 48 hours growth in presence of As III), by a silver nitrate staining test (data not shown).

However, the presence of catalysts (as prepared, calcined at 400 °C and calcined at 800 °C) degraded the Rhodamine B successfully (~95, 90 and 88 %, respectively) after three hours of exposure in presence of UV light at 554 nm.

The spheroid got attached during the first 24 hours of culture in presence of serum.