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A potential limitation of our study is the short time interval (24 to 48 hours) from initiation of antihypertensive treatment to venous blood sampling.
Blood samples were collected prior to dosing (0) and at 5, 45, 80 and 90 minutes during the infusion and 100, 115, 130, 150, 180, 210 minutes and 24 hours from initiation of infusion, and the plasma was analyzed to provide a pharmacokinetic profile (AUClast, Tmax, Cmax and Cmin).
In 4 hours from initiation of levosimendan treatment, dobutamine and dopamine infusions were stopped respectively.
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Mean BIS, SR and EMG values were then calculated per hour from initiation of TTM onwards and were used for data analysis.
These values were separated for the first 3 and last 4 days of treatment, because quinine concentrations are most likely to be in a steady state after Day 2 or 50 72 hours (terminal half-life 8 12 hours) from treatment initiation.
We classified all patients into 2-hour groups (from 0 to 12 hours) from admission to initiation of surgery and calculated the number of survivors and non-survivors and the survival rate at 60 days for each group.
Time (measured in hours) from admission to initiation of CAG, PCI or CABG was calculated using information from the NPR (the specific SKS codes can be seen in online supplementary appendix 1).
Several studies reported comparative analysis of respiratory parameters between survivors and nonsurvivors of ECCO2R [ 24, 27, 28], whilst other studies reported measurements at varying time points, from 2 hours after initiation of ECCO2R to its discontinuation.
The median duration of MV from initiation to discontinuation was 85 hours (range 1 to 345 hours), with a median time of 37 hours (range 1 to 245 hours) on PCV and 32 hours (range 0 to 264 hours) on ASB.
Kim and colleagues performed RNA sequencing on C. elegans larval samples collected every two hours from zero to 48 hours after initiation of larval development (Kim, D., et al., 2013).
Subjects were closely monitored for 8 hours from the time of the initiation of the infusion and returned after 24 hours from the time of initiation of infusion for blood sampling and additional safety assessments.
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