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Replicate serum bottles were prepared and 3 bottles were harvested every four hours for analysis.
Liquid aliquots were removed from the reactor at 0, 1, 2, 3, 6, 12, 24, and 48 hours for analysis by headspace SPME GC-MS.
Notably, plasmonic MS can be advantageous in terms of speed (reaction free, in minute overall analysis) and sensitivity (~ 6 nL of sample required), compared to the biochemical method that requires several hours for analysis (including bio-reactions and pre-treatment steps) and utilizes milliliters of sample.
Conditioned media were harvested after 24 hours for analysis.
Cells were treated with LLL12 and their media was collected after 8, 16 and 24 hours for analysis.
For analysis of the immediate early gene response in vivo, recombinant epidermal growth factor (30 mcg, Invitrogen) or vehicle (normal saline) was injected intraperitoneally into control or transgenic mice, and hearts were collected after 1.5 hours for analysis of immediate early gene expression.
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Vacuum stripping was applied to the model solution for 5 h during which samples were taken from the fermenter every hour for analysis.
75 Blood samples were taken at regular intervals up to 24 hours postdose for analysis of compound, and Aβ concentration and CSF samples were collected 4 hours after dosing.
Rats in the behavior group were placed back into the fear conditioning chamber (context A) 24 hours later for analysis of freezing to the original training context (contextual fear memory).
Cells were harvested 48 hours later for analysis using Dual Luciferase Reporter assay system (Promega, Madison, WI).
Fibroblasts were cultured for 8 hours for RNA analysis and 48 hours for protein analysis.
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