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To standardize sampling, all fish were fed ad libitum exactly four hours before sampling.
The owners indicated that most of the animals had died 12 to 72 hours before sampling.
The patients were told not to exercise 24 hours before sampling.
It was elicited by injection of prostaglandins at least 10 hours before sampling.
Older tadpoles were injected intraperitoneally with 4 6 µl of 0.05% BrdU in deionized water 24 hours before sampling.
The dogs had not been active 24 hours before the sampling, and were fasted 15 hours before sampling.
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Additionally, the dilution rate was checked manually by measuring the mass of the harvest outflow within a timespan of one hour before sampling.
Prominent kakusei expression was detected only in the forager brains, not in the follower brains (Figure 4), possibly due to the differences in their foraging experience during the hour before sampling.
The lack of "xenobiotic metabolism" pathway genes in our data might be due to the low concentration of BaP applied to Xenopus, as well as the relatively long 6-hour period before sampling at the first experimental time point.
Fresh populations of mixed stages were cultured for 96 hours at 20°C before sampling.
Urine gives short term information (few hours before sample collection).
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