Exact(6)
The illumination level was 3x lower than in Fig. 2b to limit photobleaching; indeed, no fluorescence decay was observed on a 2 hour timescale.
GRSs of 14 day-old bees fed with an unscented sucrose solution did not differ over the 48 hour timescale tested (K W: H = 0.514, p = 0.916, N = 107, Fig. 4A).
Akt activation (starting at t = 60 min and remaining active for the rest of the simulation) causes FOXO translocation to the cytoplasm; accordingly, transcription almost stops and the cytoplasmic and nuclear concentrations of mRNA fall; the level of the protein also begins to fall, although the effect is not very large on a 1 hour timescale.
So this 4 hour timescale must be something having to do with a signaling cascade specific to biological systems?
Thus, acquisition times of spectral data sets from very large lymph nodes was time consuming and in the hour timescale.
In fact, as Reviewer 3 points out, we know that this does not happen – Kog1 bodies disassemble on the hour timescale.
Similar(54)
This up-regulated expression profile was displayed at the minute and hour timescales in c2, c5, c7, and c8.
Table 6 shows that during the first 18 hours, a large proportion of the patients have an increase of insulin sensitivity using the 6-hour timescale.
However, we have chosen a 4-hour timescale to emphasize steady-state fluxes over short-term feedback, and so even though these reactions exist in the model, they are not used.
Next, acute hypoxia stimuli were applied, with the assumption that a 4-hour timescale would long enough for metabolism to adapt to a new steady state, but short enough to avoid gene expression changes.
Second, the investigation of effects on functional hemodynamic parameters is typically over a relatively short (24-hour) timescale, which may not allow drug-induced effects or feedback/disease processes over longer timescales to be observed or quantified in a systems model.
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