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When we analyzed the diurnal patterns of these genes, we found TDC3 to be under diurnal modulation with upregulation at 0 4, 20 24, and 44 48 hour time points (Figure 4B and C).
Similar effects on DNMT1 depletion were observed at 48 and 72 hour time points.
However, we observed specific accumulation of the probe in tumors at the 12 hour and 24 hour time points.
In KG-1a cells, induction of phospho-H2AX above basal levels was observed at AZA concentrations ≥1 µM at 48 and 72 hour time points.
Based on previous experiments, 2 and 8 hour time points were chosen as they represent the peaks in cytokine/chemokine levels and neutrophil influx, respectively [15], [29].
At low concentrations (<0.3 µM), very few genes were regulated at the 24 and 48 hour time points by either drug (data not shown).
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The supernatants were collected at 6- and 24-hour time points.
Cz treatment showed a dose-dependent decrease in this model as well, in both 24 and 48-hour time points.
Similar to the response seen in U2OS cells, doxorubicin did induce a significant increase in p-p65536 at both 1.5 and 3-hour time points (Figure S1).
Similar patterns were seen at the 5- and 24-hour time points (data not shown).
ZDHHC2 overexpression lasts stably at 24-, 48-, and 72-hour time points.
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