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Note the presence of residual zerovalent Ni on the surface after 24 hour reaction at 200°C.
Open image in new window Fig. 7 100 hour reaction data for the 1 wt% La-containing alumina supported bimetallic carbide catalyst.
An increase in temperature from 25°C to 37°C during the SNAPtide-conversion reaction also improved the signal somewhat (Figure 2F), and reliable readings were obtained for 1 hour reaction times.
Table 3 shows the yields obtained for partial and total hydrolysis of bagasse samples after a 48 hour reaction.
Using 3.5 nM ADAMTS13 and 2 hour reaction times, VWF106 was partially proteolysed by ADAMTS13 after 120 min (Fig. 3).
After a one hour reaction at 55°C, 500 μL of water was added and NaCl was poured until saturation.
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Reaction time, temperature and stoichiometry were the subject of detailed optimization studies, and it was ultimately determined that 1 hour reactions at 25°C with a 5∶1 molar ratio of PEG:enzyme typically yielded maximal mono-PEGylated product, i.e., protein molecules each bearing a single PEG polymer chain.
After 1 hour, reactions were terminated by the addition of sample buffer and run on SDS-PAGE gels.
Following cDNA synthesis at 42°C for 1 hour reactions were stopped by heating at 75°C for 10 min and cDNA diluted to 500 μL total volume with water.
At 70 and 22°C, both NO2- and NO3- solutions were found to be stable in experiments without metals or alloys during the 24-hour reaction period.
The results reveal that MnOx supported on P25 pretreated at 600 °C for 5 h (MnOx/P25-600-5 h) shows the best catalytic performance achieving 33.2% HMF conversion along with 97.0% selectivity to DFF in a two-hour reaction time.
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