The GD-RL group received 3 mL/kg per hour of lactated Ringer solution plus 250 mL of lactated Ringer solution if SvO2 was less than 60%.
Exclusion criteria: patients with SAH and ΔT >96 hours; patients who presented a vasospasm episode in less than 24 hours of Mg solution infusion.
After 48 and 72 hours, 10 μL of solution containing ca. 100 larvae was removed from each well for analysis and quantification of paralyzed larvae number using an optical microscope at 100x magnification.
The acyclic alkene (1 mmol) in THF (2 mL) was added dropwise, over a period of one hour, to a solution of trimethyl trichloromethyl)silane (TMSCCl3; 0.21 g, 1.1 mmol) and tetrabutylammonium triphenyldifluorosilicate (TBAT; 0.027 g, 5 mol %) in THF (2 mL) under argon at room temperature, and the mixture was stirred overnight.
The R-RL group received 3 mL/kg per hour of lactated Ringer solution throughout the entire experiment.
The GD-C group received 3 mL/kg per hour of lactated Ringer solution plus 250 mL of hydroxyethyl starch (130/0.4) if SvO2 was less than 60%.
The 'goal-directed Ringer lactate' (GD-RL) group (n = 9) received a fixed administration of 3 mL/kg per hour of lactated Ringer solution throughout the experiment.
The 'goal-directed colloid' (GD-C) group (n = 9) received a fixed administration of 3 mL/kg per hour of lactated Ringer solution throughout the experiment.
The 'restricted Ringer lactate' (R-RL) group (n = 9) received a fixed administration of 3 mL/kg per hour of lactated Ringer solution throughout the experiment without additional fluids.
The optical density is stable for several hours after solution of the formazan in DMSO.
After the mixture was stirred for 1 hour, a solution of 25 (1.00 g, 4 mmol) in CH2Cl2 (2 mL) was added into the mixture during a period of 1 hour at 0°C.
