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At 50 μg/ml of EDDHA, bacterial growth is almost completely inhibited within an hour of addition.
NEU damage also led to phosphorylation of RPA at threonine 21 residue and induced localisation of phospho-RPA proteins to nuclear foci in HeLa cells within 2 hour of addition of the drug.
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However, elongated fibrillar aggregates were observed within 8 10 hours of addition of Italian Aβ (1 40) to the lipid bilayer (Fig. 6B D).
We expected many such hits, but only RRM2 passed all our prioritization criteria, probably because cells would have had to arrest within ∼12 hours of addition of siRNA treatment, which would select for very unstable and/or dose-sensitive proteins.
Cervical epithelial cells (HeLa 229) infected with C. trachomatis serovar LGV L2 were treated with NECA, which caused a 90% reduction in reinfectious chlamydiae within a few hours of addition.
In contrast, the CPD rapidly completes tag removal within two hours of addition (Figures 3-8), since the CPD is present at a 1∶1 ratio to the target protein and poised to undergo the autocleavage reaction [5].
Cell viability was assessed fluorimetrically after 4 hours of addition of resazurin.
When phages supplemented with lysins, normal flora were effectively controlled but the lysogens were formed after 24 hours of addition as the lysins were used along with phages.
The ferritin increase occurred within 48 hours of addition of 200 μM FAC, was dfo-sensitive, and was maintained for at least 12 weeks of culture in FAC, the longest time point examined (not shown).
Monitoring by H NMR spectroscopy at room temperature shows that after two hours of addition of phen only 81%% of the original copper(I) amide remained and after 18 h, just 46%% copper(I) amide remained with Cu also now present.
Indeed, viability assays revealed that the cytotoxic effect occurred within an hour of nanopore addition.
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