24 hours after transfection, cells were stimulated with mouse IFN-alpha (PBL interferon source) for 4 hours before RNA was isolated and profiled for alterations in IFN-inducible genes.
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Development of pigment was visually monitored from 24 48 hours for presence of melanophores or alterations in melanophore distribution (xanthophores and iridophores were not examined).
Densitometry analyses from a total of six independent experiments indicated that treatment of these cells with 0.5, 1, 1.5 or 2 mM H2O2 for 24 hours caused an alteration of band intensity to 76%, 108%, 113% or 104% of controls, respectively.
Variations in the time for which RRT is applied (3-4 hours for IHD, 6-12 hours for SLEDD, and 24 hours for CRRT) and alterations in the operational characteristics to harness diffusive and convective clearance distinguish the different modalities.
