Sentence examples for hour blocking from inspiring English sources

Reina was in action again on the hour, blocking Richards' header from Nasri's corner, prompting a change from Dalglish as he introduced defender Jose Enrique for Downing.

Additional washes in PBS preceded a one hour blocking step in 10% normal horse serum and 0.3% Triton X-100.

After a one hour blocking period in PBS (0.05% Tween, 5% powdered skim milk) blots were incubated overnight with primary antibody, washed three times with PBS and subsequently incubated with a peroxidase coupled secondary antibody.

Hypothalamic neurospheres and differentiated hypothalamic neurons culture were fixed with 4% paraformaldehyde (Sigma) and permeabilized with 1% Triton X-100 (Sigma) followed by one hour blocking with 3% BSA (Sigma) and incubation with the primary antibody overnight at 4°C.

Additional washes in PBS preceded a one hour blocking step in 10% normal goat serum (NGS: LAMPIRE Biological Laboratories, Inc. Pipersville, PA; Cat no. 7332500) and 0.3% Triton X-100.

The plates were warmed at 37°C for one hour, blocking solution (1% BSA/PBS) removed and washed twice in binding buffer (RPMI 1640 in 2% glucose) prior to adding 1.25 ml of parasite cultures at 3% parasitaemia, which had been washed twice in binding buffer and resuspended to 1% haematocrit.

For the past three days, student protesters had occupied the main highway through Caracas for several hours, blocking traffic to press their demands.

The site was cut off for several hours, blocking all users from logging in.

After a few boring hours blocking traffic at parliament, the protesters moved onto Downing Street and blocked the entrance forcing the police to arrest them for aggravated trespass.

After a one-hour blocking period in TBS-0.1% Tween-20 containing 5% skim milk, the membranes were incubated overnight with one of the following specific antibodies: anti-phospho-JNK, anti-JNK (Cell Signaling, Pickering, Ontario, Canada), anti-phospho-Syk (Upstate Biotechnology, Lake Placid, NY) or anti-Syk (Santa Cruz Biotechnology).

After a 1-hour blocking with 0.2% Triton X-100 and 3% goat serum in PBS, the cells were incubated with primary antibodies overnight at 4°C.