Sentence examples for hour blocked from inspiring English sources

After washing the cells again with PBS, we permeabilized the cells with 0.1% Tween 20 in PBS for 1 hour, blocked the cells with PBS containing 2% normal serum for 1 hour, and incubated the cells with primary antibodies for 2 hours at room temperature.

Briefly, cells were fixed with 4% paraformaldehyde for half an hour, blocked in blocking buffer (5% goat serum, 1% BSA, 0.1% Triton X-100) for 1 hour followed by incubation with the primary antibody at 4°C overnight.

Peptides were then transferred to Hybond-P PVDF membranes (Amersham Biosciences UK Limited, Bucks, UK), at 90 V for 1 hour, blocked for 40 minutes in blocking solution (5% fat-free milk in PBT) and probed overnight at 4°C with the anti-tsetse saliva rabbit anti-serum [ 27] diluted 1 7,500.

Additional washes in PBS preceded a one hour blocking step in 10% normal horse serum and 0.3% Triton X-100.

She got a ride to a border town, where she spent an anxious 24 hours, blocked by the snow.

Sose remained nearly stationary for a period of 24 hours, blocked by a strong subtropical ridge to the south.

The site was cut off for several hours, blocking all users from logging in.

After a few boring hours blocking traffic at parliament, the protesters moved onto Downing Street and blocked the entrance forcing the police to arrest them for aggravated trespass.

MMM begins a 7.5 hour block of continuous jazz, WKCR's longest in any style.

Three hour block(s) of time are required during weekdays for this project.

The prime-time portions of the Republican Convention sessions are self-contained one hour blocks.