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In each case we experimented with the program for well over an hour before testing it by transcribing three lines from T.S. Eliot's "The Love Song of J. Alfred Prufrock".
Plasma sample were centrifuged at 23,000 g at 4°C for 1 hour before testing.
Current smokers refrained from smoking for 1 hour before testing.
Subjects avoided eating, drinking, smoking, and/or exercising for at least 1 hour before testing.
Animals were brought into the testing area a minimum of 30 min (but ideally 45 min to an hour) before testing and allowed to acclimate.
One hour before testing, animals were trained on the RotaRod set at 16 rpm until they could stay on for 30 s.
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(21 °C. ±2 °C). and 55%±5%% humidity for at least 24 hours before testing.
For all salt chemotaxis experiments, gradients were allowed to develop in the agar for 3 5 hours before testing.
Films were cut into 25 × 100 mm strips and then equilibrated in desiccators over saturated salt solutions having the desired relative humidity 34% (MgCl2) and 54% RH (Mg(NO3 2) condition at 25°C for 48 hours before testing.
Twenty four hours before testing 30 flies per genotype were randomly selected under CO2 and placed in a fresh vial with standard food.
Mice are habituated to the room 24 hours before testing.
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