Sentence examples for hot started from inspiring English sources

By the time [the club night] Hot started in May '88, the roof had lifted off the Haçi.

On Fridays and Saturdays, then Wednesdays, when Hot started, there were queues of kids in painters' jeans and sweatshirts, snaking round the corner from Whitworth Street back towards G-Mex, waiting to be let into the biggest, maddest youth club in the world.

Reactions were hot started 95°C for 5' before the addition of 1.5 units of Taq polymerase (ROCHE, GmbH, Germany).

Reactions were hot started with 1 unit of Taq polymerase (Promega) and run with conditions of denaturation at 95°C for 2 min, followed by cycling at 95°C (30 s), 60°C (15 s), 72°C (30 s), with final elongation at 72°C (5 min).

Pyrosequencing reactions were hot started at 94°C for 5 minutes, and amplification was carried out over 45 cycles (30 s at 94°C, 30 s at 58°C, 30 s at 72°C), followed by 5 minutes at 72°C with sequencing primers on the PSQ HS 96A System (Biotage AB, Uppsala, Sweden) according to the manufacturer's specifications.

Before the game, Collins acknowledged the plight of overseeing a club with hot starting pitching.

If they're still hot, start the cooling on the countertop.

What's hot: Start your trip planning here to save you lots of time.

Other assays perform best with a pre-amplification, high-temperature denaturation step ("hot start").

If you feel too hot, start adding cold water into the tub until you cool off.

A hot start guarantees only higher expectations.