For each predicted pathway in the HOT dataset, a weighted taxonomic distance (WTD) distance was calculated using the WTD algorithm (Additional file 1: Supplementary Note 2).
Interestingly, the subsampled 25 m HOT dataset exhibited a 28% reduction (425 compared to 593) in pathway recovery with and without pruning suggesting that increased sample complexity can partially offset taxon specific sensitivity losses.
The results show that our proposed approach performs satisfactory to extract hot topics for datasets of both the educational blog and the "parent child" forum.
Currently, there are only two published acidic hot spring metagenome datasets [ 17, 18], one from Yellowstone National Park and the other from El Coquito spring, National Natural Park Los Nevados.
Figure 3 shows a dendrogram derived from combining the Bison Pool [ 24], Guerrero Negro [ 3] and HOT-ALOHA [ 2] datasets with a hydrothermal sediment metagenome from Great Boiling Spring (GBS), Nevada, USA [ 26] and twenty additional metagenomes from Yellowstone National Park (YNP) [ 27- 29, 25].
The presence of many taxonomically unsolved sequences in the 16S rRNA gene tag datasets from these hot springs could be a sign of novel microbe richness in these less known hot water bodies of Northeastern India.
Monitoring sites must be in focal marine areas that already have long datasets, are biological hot spots (e.g., marginal ice zones, polynyas, boundaries, and fronts), and are key to biogeochemical properties, biota, and invasive species.
The analysis and modeling of networked datasets are probably the hottest research topics within the modern science of complex systems [1] [7].
Due to the unavailability of dose homogeneity and hot spot data for the complete dataset, we were unable to test this for the total patient population, but the hypothesis is confirmed in a subset of the population [ 19].
