Like Lynda, the startup takes video quality seriously and has become a production operation just as much as a virtual video (hosting) library, developing its own studios in Seattle and San Francisco.
To estimate the extent of contamination of host libraries from symbiont RNA, we performed a tblastx search of the coral unigenes against a Symbiodinium sequence database, consisting of 2002 nucleotide sequences available in GenBank for the genus Symbiodnium.
As a comparison, we show the abundance of the two well characterized (positive-sense) RNA viruses: Japanese encephalitis virus and Gill-associated virus found in the Mosquito-Hubei and Shrimp libraries, respectively, as well as the range of abundance of host mitochondrial COI genes in these same multi-host libraries.
Despite apparent symbiont contamination in the host library, >85% of carbohydrate active sequences from termite gut library had significant homology to translated genes of animal origin.
Additionally, no laccases or other potential lignase enzymes were identified in R. speratus hindgut symbiont library as they were here from the R. flavipes host library (see below).
We present three sets of analyses with JH treatment which show enrichment of GO- Molecular Function terms in the sequences that were downregulated from both host and symbiont libraries and upregulated from the host library.
The 5,871 ESTs produced from the host library assembled into 875 contigs and 2,169 singlets to produce 3,044 putatively unique transcripts.
BLAST analyses demonstrated that all genes listed in this category have homologues in insects, and they were identified exclusively in the host library, supporting that they are endogenous to the host genome.
The gene expression profile of the host termite gut cells is not characterized by an overly abundant domain; however, this may be related to the fact that the host library was normalized.
Second, a diverse complement of previously unknown genes that encode proteins with homology to lignase, antioxidant, and detoxification enzymes were identified exclusively from the host library (laccase, catalase, peroxidase, superoxide dismutase, carboxylesterase, cytochrome P450).
