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The capacity of the multiplex RT-qPCR assay in detecting the three vitiviruses in mixed infections from a range of virus concentrations in the host was assessed.
Similarly, the observed number of miRNAs predicted to target their own host was assessed by exchanging host genes for randomly chosen genes from predicted targets and recalculation of the number of miRNAs predicted to target their host.
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Traditional means for determining mosquito host-preferences in the field are to estimate the proportion of blood meals on certain hosts [1] or, more specifically, to calculate a feeding index, where the proportion of blood meals acquired from a specific host is assessed in relation to the abundance of that host within the community of potential hosts [2], [3].
The frequency of allogeneic cells found in BM and in the spleens of SHIPi or vehicle conditioned hosts was assessed by flow cytometry.
Differences between sibling species in prevalence, the proportion of hosts infected with at least one larval Arrenurus, and intensity, the mean number of Arrenurus on only the infected hosts, was assessed in QP3.0 [ 24].
Given the contradictory results of different studies, the potential role of humoral immune responses for the control of viral replication in natural hosts was assessed in AGMs by ablating anti-SIV antibody production through a long-term depletion of B cells.
The symbiotic effects on the vegetative growth of the hosts were assessed by total increased fresh weight and dry biomass of the plantlets.
Host tolerance was assessed by plotting the relative host plant biomass of infected plants (i.e. the biomass of parasite infected plants as percentage of the biomass of parasite-free control plants) against the Striga infection level, expressed as the total biomass of the parasitizing plants collected from the host roots.
The virulence of B. pseudomallei natural isolates in the C. elegans host model was assessed by measuring the survival of worms fed on pure cultures of these isolates.
The effect of parasite infection on the lipid domain fraction of host erythrocytes was assessed by preparing lipid domain fractions according to an established method [ 27, 41].
Cyto-adherence of Mmm and Mmc to specific host cells was assessed by testing the binding ability of both Mycoplasma species to goat and cattle cells.
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