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Similarly, HNF4α is required for HBV host viability.
We re-tested the identified targets after exclusion of genes that affect host viability and transcription/translational processes [32].
The dependency of HBV transcription on HNF4α links viral biosynthesis and persistence to a developmentally regulated transcription factor essential for host viability.
Our goal was to engineer the pathogenicity of the virus to only interfere with host viability on the basis of overactivation of the Ras/ERK/ELK pathway.
Additionally, the essential nature of the HNF4α transcription factor for host viability [10] [12] may limit the hosts' ability to resolve infection and increase the probability of viral persistence.
It was therefore impossible to remove pAMI5, which strongly suggests that this replicon might be essential to host viability.
Similar(42)
Rewardingly, chalcones 8o and 8p (at 10 μM) each gave >92% inhibition of viral propagation without impacting PM1 host cell viability.
These studies begin to unravel requirements for robust virus replication and the relationship between increased virion production and host cell viability.
However, engineering metabolic pathways within a microbe is a complicated process: levels of gene expression, protein stability, enzyme activity, and metabolic flux must be balanced for high productivity without compromising host cell viability.
Whenever reduced host cell viability was observed, it did not translate into reduced production of virions.
Biotrophic fungi derive their energy from the living cells of their plant hosts without loss of host cell viability.
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