RMDAP contains donor and recipient vectors, and uses bacterial strain SW106 as the recipient host, which has all the requisite in vivo recombination proteins, including an arabinose-inducible cre gene and a defective λ prophage with recombination proteins exo, bet, and gam controlled by the temperature-sensitive repressor cI857 [17].
Just like the Barbara Walters show it is skewering, Daytime Divas's fictional talk show features the requisite conservative blonde host, Heather Flynn-Kellogg (played by Fiona Gubelmann), who, as the show's opening titles tell us, is "a real slice of American pie who puts faith in family first".
After Whiteley's untimely death in 2005, qualifying to hold the host spot on Countdown has been largely contingent upon having the requisite "chemistry with Carol" factor.
9 P.M. (CMT) JENNIE GARTH: A LITTLE BIT COUNTRY Ms. Garth hosts a barbecue for her mother and new stepfather with the requisite bluegrass band and a mechanical bull.
8 P.M. (Fox) SO YOU THINK YOU CAN DANCE Season 5 begins with two hours of the requisite audition rounds, judged by Nigel Lythgoe and Mary Murphy; Cat Deeley is the host.
Collectively, these modifications in step 1b allow for the requisite assembly of evidence of projected harms and consideration of a host of associated methodological concerns related to institutional adaptive capacity (e.g., Gosling et al. 2012; Huang et al. 2011).
At the root of these successes is the identification of the requisite pathways and the systematic transfer of these pathways to a microbial host.
(The way it worked was that first, everyone on Twitter made the requisite Alvin Greene jokes, before moving on to talking about the Comedy Central host's certain riff on the matter).
Common microbial hosts such as E. coli can produce high yields of recombinant protein but lack the requisite machinery for post-translational modification required for protein stability and bioactivity [ 26].
This latter demand leads to a host of other requirements: in order to be convinced of something new the opponent must have the requisite doubt, understand the terms and accept the subject of debate.
