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It is therefore necessary to develop genetic parts that are functional regardless of host strains.
Some aquatic birds are known to host strains of the virus with no or minimal symptoms.
Various cultivation strategies employing different host strains and expression systems have been successfully employed for the production of recombinant proteins.
For heterologous gene expression in E. coli a number of different expression vectors and host strains are used.
Fig. 4 Kinetics of α-amylase from C. flavus production by recombinant S. cerevisiae JPU and CEN.PK2 host strains.
To check isobutanol tolerance the host strains were cultured in liquid medium in the presence of increasing concentration of isobutanol.
Examples range from therapeutic antibodies, to rationally engineered (synthetic biology) host strains, to stem cells for therapy.
We therefore re-engineered the TFIIE subunit production vectors and tested various Escherichia coli host strains to optimize expression.
Phylogenetic analysis suggests that CYP102A1 gene could have evolved more rapidly than the rRNA gene locus of the host strains under the selective pressures of their environments.
In recent years, the advancement of tolerance engineering and evolutionary engineering provides powerful platform for obtaining host strains with desired tolerance for further metabolic engineering of biofuel pathways.
Additionally, there is a large number of available cloning vectors and several mutant host strains with differing characteristics that increase the likelihood of obtaining soluble protein.
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