Sentence examples for host passage from inspiring English sources

It is thus tempting to propose that apoptotic death progresses during metacyclogenesis in the vector and that due to the temperature change occurring during insect to mammalian host passage, the number of PS-exposing promastigotes increases at the inoculation site.

The requirement of host passage and lack of in vitro culture for most parasitic nematodes means that one will most likely need to identify knockins through scoring a selectable cointegration marker (i.e., drug resistance or fluorescent markers).

We used parasite genotypes from stabilates frozen as close as possible to the date of collection/arrival in Edinburgh (dates in Additional file 1) to avoid genotypes previously subjected to artificial selective pressures (intentionally or unintentionally) and minimize host passages.

The longer lifecycles and potential requirement for multiple host passages to obtain stable knockins poses a significant challenge to implementing these tools, which may be insurmountable for many parasites.

Manipulations and host cell passage histories are expanded in the Detailed Materials and Methods (Appendix S1).

Even a virus-immune vertebrate host permits passage of that virus between simultaneously feeding vector ticks (7).

Nonetheless, an ideal system would be one that remains inactive when administered to the host, survives passage to the GI tract and then produces the proteins of interest only when needed, for example, only in the presence of pathogens.

Low host density passages were initiated by adding 104 mutagenized phages to 100 mL containing 106 cells each of E. coli and S. typhimurium (106 bacteria/mL for each host type).

High host density passages were initiated by adding 104 mutagenized phages to 1 mL of LC containing 108 cells each of E. coli and S. typhimurium (108 bacteria/mL for each host type).

Previous studies with both alpahviruses [16], [17] [19] and flaviviruses [11], [20] have demonstrated host specialization with passage, yet previous passage of SLEV in Cx. pipiens demonstrated a lack of adaptation in Cx. pipiens [21].

Parasites were maintained by serial passages in MEF, harvested from infected host cells by passage through a 26-gauge needle and purified by separation on Sephadex-G25 columns (Amersham) as described [62].