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The complex life cycle of the human malaria parasite P. falciparum, divided between insect vector and human host, necessitates dynamic protein expression and gene regulation to exploit efficaciously the different host environments.
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Therefore, engineering cell-based devices that interface robustly with host physiology necessitates new technologies for engineering cell-surface biosensors that transduce the detection of exclusively extracellular ligands into changes in cell state.
Understanding the epidemiology of host-pathogen dynamics necessitates an appreciation of both the within-host [26], [27] and between host [37] dynamics.
The ability of Lyme disease borreliae to perpetuate their natural vertebrate-tick infectious cycle requires an array of strategies to survive in diverse host environments, and necessitates mechanisms to overcome innate and adaptive immune responses of several hosts.
The expressed product remained within the host cell and necessitates complicated purification methodologies.
Like pathogens, ECM fungi are obligately dependent on plants and the decline of one host species may necessitate the switch to another (Raffaele and Kamoun 2012).
Further, the mobile code environment is fluid, i.e. the programs and resources located on a host may change rapidly, necessitating an extensible security model.
The infection of an implanted prosthetic material can have serious consequences on the tissue integration of the implant and the scarring process in the host, and may even necessitate replacement of the prosthesis.
Persistent viral infections necessitate lifelong host immunity.
As these proteins could be involved in ruminant strain host tropism, their functions necessitate further exploration.
However, dealing with mammalian genomes and a large number of sequencing reads (>10 million) necessitates a host-based sequence subtraction step for increasing accuracy.
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